EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler

Quick Details

The EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler EKONPCR03 system adapts to almost any workflow requirement with flexible instrument control and data management.

>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands

>> High-throughput up to 96 samples per run

>> Push-pull, electrical controlled elevating platform

>> Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature control

>> Quick-start set-up so you can begin a run immediately

>> Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.

>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.

1. Co-located configuration (PC control)

2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)

3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)

Specifications

EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler

The EKONPCR03 system adapts to almost any workflow requirement with flexible instrument control and data management.

>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands

>> High-throughput up to 96 samples per run

>> Push-pull, electrical controlled elevating platform

>> Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature control

>> Quick-start set-up so you can begin a run immediately

>> Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.

>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.

1. Co-located configuration (PC control)

2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)

3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)

EKONPCR03 Real Time fluorescence quantitative PCR System

EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler

Fast and uniform heating and heat sinking block

>> Concealed lid with constant temperature and isopiestic pressure

>> Electrical controlled elevating & automatic locked platform

>>Thermo block is based on improved heat pipe technology, leading to extraordinary Iintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.

>> Highly efficient PID&DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking

H igh sensitiv e and precise optical system

>> High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could be from 10 1 to 10 10 copies

>> Fast and precise mechanical scanning structure ensures the illumination uniformity of 96 wells, Eliminate edge effects, no need for ROX calibration

>> Fluorescence excitation by a high-intensity and long-life LED, Bulb Luminance will not attenuation over time, free maintenance optical system through the entire life.

>> Professional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.

EKONPCR03 Real Time fluorescence quantitative PCR System

EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler Easy- to - use Software

The EKONPCR03 Software offers an intuitive experience for protocols programming, plate layouts setting and experimental results analysising. The Software includes an unlimited license for multiple users. The available software modules include Absolute quantification, Melting curve analysis, Relative quantification and High Resolution Melting (HRM). All the excellent features make the instrument to your ideal choice for qPCR.

>> Friendly interface and clearly functional modules design, first-time users can easily start a new experiment.

>> All experiment data and setting options are shown conveniently on a single interface. User modifications to analysis parameters can be seen instantly

>> Powerful analytical capabilities, optional automatic and manual threshold setting bring reliable date you can work on.

>> Experimental date can be export in.csv,.excel,.txt format, with this capability, the EKONPCR03 can interface with your laboratory information system directly.

>> Unique designed universal report can be customized according to the requirements, report review and print function help clinical documentation as needed.

This real-time PCR run was done using 5 ng of hepatitis B virus DNA in each of the 96 block positions. (A) A 135 bp amplicon of the hepatitis B virus gene was amplified using SYBR Green I detection. (B) The reaction product was also subjected to melting curve analysis. In summary, low variation Cq values (Cq range = 0.15and SD = 0.040) and overlapping melt curves of the amplicon (T m range of 0.5°C and SD = 0.065) in each of the 96 positions demonstrated temperature homogeneity and equal treatment of all samples - independent of block position.

A hepatitis B virus gene fragment was amplified in ten serial 1:5 dilutions ranging from 108 to 100 copies per well. Eight replicates were run for each dilution. Results on the EKONPCR03 System show excellent reproducibility and resolution down to very low copy numbers.

EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler Specifications

Thermal Block

Block formats 96-well

Sample volume 5–50 μL

Consumables 0.1ml 96-well unskirted Plates; 0.1ml 8-strip tubes (Optical flat cap)

Max heating/cooling rate 4.2°C/sec

Temperature range 4.0–99.0°C

Temperature accuracy ≤±0.1℃

Temperature uniformity ≤ ±0.3°C

Temperature steps 1-99

Heated lid

Temperature range 100–120°C

Control Automatic temperature and pressure setting

Optics

Excitation 4 LEDs

Excitation range 470–630 nm

Detector Photodiode

Detection range 525–670 nm

Detection channels:

Channel Excitation (nm) Emission (nm) Pre-Calibrated Dyes

1. 470 525 FAM, SYBR Green I

2. 523 564 VIC, HEX, TET, JOE

3. 571 621 ROX, TEXAS-RED

4. 630 670 CY5

Multiplex Up to 4 targets

Detection range 101-1010 copies

EKONPCR03 Real Time fluorescence quantitative PCR System thermo cycler Software

Analysis modes: Absolute quantification, Relative quantification,

Melt curve analysis, High Resolution Melting

Performance

Reproducibility CV≤2.1％

Correlation coefficient of nucleic acid precision R≥0.997

System

Operating systems Windows XP

Communication Ethernet or USB

Power usage AC220 × (1±10%)V, 50 × (1±2%)Hz

Dimensions 540W×300D×480H mm

Weight 35kg

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.