Real-time Quantitative PCR Detection System thermal cycler EKONPCR02

Quick Details

Real-time Quantitative PCR Detection System thermal cycler EKONPCR02 Features

1.48 samples

2.Easy-to-use Software

3.Various Operation modes

4.Advantange Temperature Control

5.Non-Maintenance Optical Systems

6.Sensitive and Precise Thermoelectric Systems

7.Particular Momentary Power Failure Protection

Specifications

Real-time Quantitative PCR Detection System thermal cycler EKONPCR02

Key Features

1.48 samples

2.Easy-to-use Software

3.Various Operation modes

4.Advantange Temperature Control

5.Non-Maintenance Optical Systems

6.Sensitive and Precise Thermoelectric Systems

7.Particular Momentary Power Failure Protection

Real-time Quantitative PCR Detection System thermal cycler EKONPCR02 Specifications

Thermal Block

Block formats 48-well

Sample volume 10–100 μL

Consumables Thick-walled 0.2ml PCR tibes, 0.1ml 8-strip tubes

Max heating/cooling rate 3.0°C/sec/ 2.0°C/sec

Temperature range 4.0–99.0°C

Temperature accuracy ±0.1℃

Temperature uniformity ±0.3°C

Temperature steps 1-99

Heated lid

Temperature range 100–120°C

Real-time Quantitative PCR Detection System thermal cycler EKONPCR02

Optics

Excitation 2 LEDs

Excitation range 470–523 nm

Detector PMT

Detection range 525–564 nm

Detection channels:

Channel Excitation (nm) Emission (nm) Pre-Calibrated Dyes

1. 470 525 FAM, SYBR Green I

2. 523 564 VIC, HEX, TET, JOE

Detection range 10 1 -10 10 copies

Software

Analysis modes: Absolute quantification, Relative quantification, Melt curve analysis,

Performance

Reproducibility CV≤2.1％

Correlation coefficient of nucleic acid precision R≥0.997

Real-time Quantitative PCR Detection System thermal cycler EKONPCR02 System

Operating systems Windows XP,Windows 7

Communication USB

Power usage AC220 × (1±10%)V, 50 × (1±2%)Hz

Dimensions 520W×420D×320H mm

Weight 27kg

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.